MetaChat - Holy Pointlessness Batman!

MetaChat is an informal place for MeFites to touch base and post, discuss and chatter about topics that may not belong on MetaFilter. Questions? Check the FAQ. Please note: This is important.

11 March 2006

Holy Pointlessness Batman! →[More:]So, I just drove for 7 hours (almost 1000 km) to the FedEx depot in Mississauga and back for no reason. The FedEx depot in my city isn't open on Saturdays (!!) so I had to go pick up my package. What was in the package? A cell line I requested from a lab at UCLA....dead...all dead. UCLA packaged the cells in the wrong box and so the dry ice they were on had sublimated (a long time before I got my hands on it I would say). So I drove for 7 hours to pick up dead cells. Did I mention gas is not cheap? Yeah...it isn't.

Tell me funny things to help me feel better, please?

post by: LunaticFringe at: 18:16 | 14 comments

My almost two year old daughter woke me up this morning by urinating on my feet. I was asleep in my chair and she walked into the room, pulled off her diaper and pissed on my feet. She laughed and laughed and laughed.

That's a hell of a way to wake up.

posted by ColdChef 11 March | 18:21

I would not want to wake up that way. I hear things like this and it makes me scared of kids! My day kinda sucked but at least no one peed on my feet....

posted by LunaticFringe 11 March | 18:25

My day kinda sucked but at least no one peed on my feet....

I think I have a new mantra.

posted by mudpuppie 11 March | 18:31

(certain) Cell lines can be pretty robust. Why not throw them in some complete media in case a few managed to survive by munching their sibling's liver or something?

As for dumb shit, I decided, hey, wth, and clicked on dgaicun's link.

(note, don't click on the link in the link)

posted by porpoise 11 March | 18:43

I've plated them just in case some survived but I'm not holding my breath. I'll know tomorrow for certain. Anybody work with MEFs? Are they hardy?

posted by LunaticFringe 11 March | 18:45

I clicked it...I don't know what to say about it.

posted by LunaticFringe 11 March | 18:48

Hey! Keep the tip!

Damn you.

posted by ColdChef 11 March | 18:51

Mouse embryonic fibroblasts? I assume that they were in frozen glycerol stock, shipped frozen... depending on how well they were frozen, there might be a few that're still viable. They tend to be relatively robust - because the (potential) titre is so low it might take a while to reach near-/confluence.

Unless they've got something wierd transfected in them or something, then all bets are off.

Or are you referring to a Marine Expeditionary Force? Those fuckers are tough as nails, they'll be ok.

posted by porpoise 11 March | 19:57

You people are ruining my hair.

posted by chewatadistance 11 March | 20:09

You people are ruining my hair.
Sorry. :( I'll stop now
*stop everything*

They're mouse embryonic fibroblasts that have GFP embedded on the inactive X. I have no idea how hardy these guys are. I'm keeping my fingers crossed though.

I don't even know what the Marine Epeditionary force is. Sounds hardy though.

posted by LunaticFringe 11 March | 20:33

Marine Expeditionary Force

I guess if it's not actively over-expressing something that does something it won't really make a difference. Good luck on those cells and I hope there's someone (the shipper) that you get to scream at a little.

posted by porpoise 11 March | 20:47

I like the sciencey talk and all, it just goes over my head at high speed. :)

posted by chewatadistance 12 March | 07:42

At least it's not engineerey talk...

posted by porpoise 12 March | 09:14

I worked in hospitals and with labs and so i kinda get what porpoise blew out of his porthole with that. Personally, I think any phrase that contains the word "mouse", and any other scientific/biological jargon is neato. I like the word mouse.

posted by Lipstick Thespian 12 March | 14:23

← Music Box -- On-the-fly records || Testing the Gullibility of MeFites →

HOME || REGISTER || LOGIN